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Sample Preparation and Withdrawal of GSH from PC-12 Cells Cells (1 10 6 cells/sample) were cultured in DMEM supplemented with 10% FBS at 37C for 24 h, followed by treatment with either MnCl 2 (5 mM), CoCl 2 (5 mM), methylglyoxal (0.4 mM), or hydrogen peroxide (0.1%) for additional 24 h